DeltaVision Elite with Phase Contrast optics
Inverted Widefield fluorescence deconvolution microscope.
- Environmental control chamber with CO2 and temperature control.
Lenses
| Objective | NA | Tech | WD/mm | Imm. | PFS | Material Ref. |
|---|---|---|---|---|---|---|
| 10X U-Plan S-Apo | 0.4 | 3.1 | Air | Y | 1-U2B823 | |
| 20X U-Plan FL LWD | 0.45 | Ph1 | 6.6 | Air | Y | 1-U2B375 |
| 40X UApo | 1.30 | 0.1 | Oil | Y | 1-UB768 | |
| 60X Plan Apo N | 1.42 | DIC | 0.15 | Oil | U | 1-U2B933 |
| 60X LCPLPL | 0.7 | Ph2 | 0.15 | Air | Y | 1-UC351 |
| 100X Plan Apo | 1.4 | Ph3 | 0.13 | Oil | Y | 1-U2B836 |
Filters
There are three polychroics available. Within each polychroic, the four excitation and emission filters can be independently chosen.
D-T-F-C
| Filter | Ex/nm | Em/nm |
|---|---|---|
| DAPI | 381-412 | 420-456 |
| FITC | 464-492 | 500-523 |
| TRITC | 531-565 | 573-611 |
| Cy5 | 619-646 | 654-700 |
D-F-mCh-C
| Filter | Ex/nm | Em/nm |
|---|---|---|
| DAPI | 381-412 | 420-456 |
| FITC | 464-492 | 500-549 |
| mCherry | 557-590 | 598-617 |
| Cy5 | 625-646 | 654-700 |
C-Y-mCh
| Filter | Ex/Nm | Em/nm |
|---|---|---|
| CFP | 400-453 | 463-487 |
| YFP | 497-527 | 537-549 |
| mCherry | 557-592 | 602-662 |