Nikon Ti
Nikon ti microscope

Inverted wide field fluorescence microscope

 

  • Phase contrast and DIC objectives with Perfect Focus System™
  • A Nikon Qi2 16.2 megapixel monochrome CCD camera as well and a EMCCD cameras suitable for live-cell imaging.
  • Environmental chamber with both temperature and CO_2 control.
  • Filter sets include: mCherry (HC Red), GFP, YFP, Texas Red, Cy5.5, DAPI, UV and TRITC.

Objectives

Objectives NA Tech WD/mm Imm. PFS Material Ref. link
4X Plan Apo 𝝺 0.2 BF 20 Air Y MRD00045 link
10X Plan Fluor DLL 0.3 Ph1 16 Air Y MRH10101 link
20X S Plan Fluor ELWD 0.45 Ph1 8.20-6.90 Air Y MRH48230 link
40X S Plan Fluor ELWD 0.6 Ph2 3.60-2.80 Air Y MRH48230 link
100X Plan Apo VC 1.4 DIC 0.13 Oil Y MRD01901 link
100X Plan Apo 𝝺 1.45 Ph3 0.13 Oil Y MRD31905 link

Standard Filters Cubes

Name Ex Em Position
DAPI 340-380 435-485 2
CFPHQ 420-445 460-510  
GFP-B 460-500 510-560 6
Texas Red 540-580 600-660  
CFL-mCherry 550-590 608-683 3
mCherryHQ 550-590 607-682  
Cy5.5 635-675 695-735  

Polychroics

BGRFR quad (position 4)

Name Ex Em
DAPI 380-395 405-470
FITC 472-500 505-530
TRITC 545-575 585-625
Cy5 640-650 672-730

CFP-YFP dual (position 5)

Name Ex Em
CFP 400-434 450-480
YFP 490-515 525-570

 

Acknowledgement of Country

UTS acknowledges the Gadigal people of the Eora Nation, the Boorooberongal people of the Dharug Nation, the Bidiagal people and the Gamaygal people, upon whose ancestral lands our university stands. We would also like to pay respect to the Elders both past and present, acknowledging them as the traditional custodians of knowledge for these lands.